(Current Challenges and Updates in Pharmacy Education and Research)

B-sitosterol and stigmasterol products of aerial part lactuca serriola by combiflashNextgen and HPLC

Not scheduled

20m

كلية الصيدلة

Recorded poster Conference Track Three

Speaker

Ms Murtadha Alnaili (College of Pharmacy University of Baghdad )

Description

Abstract
Purpose: the work aimed at isolation and characterization some bioactive constituent(s)from aerial part of lactuca serriola hance by CombiflashNEXTGEN and high performance liquid chromatography (HPLC).
Method: lactuca serriola was extracted with hexan and then 250mg of hexan extract mix with 4g slica gel and loaded in cartilage then bounded to gold colum of comi flash using a solvent system comprised of ethyl acetate: n-hexan (10%ethyl acetate 90%hexanv/v) used to elute the column successively .The fractions result analysis by thin layer chromatography and high performance liquid chromatography (HPLC).
Results: column chromatography which resulted in the isolation and purification of a white needle-like crystalline substance analysis by thin layer chromatography and high performance liquid chromatography (HPLC). Including b-sitosterol(11mg) and stigmasterol(7mg) , were obtained from 250 mg of hexan fraction.

Conclusion: by CombiflashNEXTGEN is a rapid and effective method for isolating and purifying plant sterol,(b-sitosterol and stigmasterol).
Keywords:, CombiflashNEXTGEN colum chromatography,lactuca serriola, b-sitosterol, stigmasterol.