(Current Challenges and Updates in Pharmacy Education and Research)

The Evaluation of the effect of Receptor of Advanced Glycation End Products-Gene Polymorphism on Aspirin Resistance in Coronary Artery Disease Iraqi Patients with and without Type 2 Diabetes.

Not scheduled

20m

كلية الصيدلة

Recorded poster Conference Trak Two

Speaker

falh abd

Description

Objectives: the aims of this study are to evaluate the association of Aspirin resistance with Receptor of Advanced Glycation End product (RAGE) – gene polymorphism in coronary artery disease Iraqi patients with and without diabetes type 2 and to detect the prevalence of Aspirin resistance in studied population.
Patients and method: From February 2021 to October 2021, apparently healthy participants 130(97 males,33 females) not taking Aspirin served as control group and coronary artery disease (CAD) patients of 232 (166 males, 66 females) already they were on aspirin 100 mg as prophylaxis were enrolled in a cross-sectional study.
The response to Aspirin is evaluated by measurement the serum level of thromboxane B2 (TBX2), which is the more stable and measurable metabolite due activity of cyclooxygenase-1 enzyme that directly inhibited by Aspirin. Accordingly the patients were divided into two groups: sensitive and resistant to aspirin.
Through polymerase chain reaction amplification of the
extracted deoxyribonucleic acid, and sequencing by Sanger method to identify the polymorphism of mostly related single nucleotide polymorphisms (SNPs) of RAGE (rs1035798 & rs184003).
Results: The total prevalence of aspirin resistance was 17.8%, for CAD patients without DM was 16.8%, while for (CAD) patients with (DM) the prevalence was relatively higher (18.9%).
For both rs184003 and rs1035798, the wild type (CC) genotype was found to be significantly lower in resistant groups as compared to sensitive group, but there was significantly higher frequency of heterozygous (CT) genotype in resistant group compared to the sensitive one (p< 0.05).
Conclusion: The prevalence of aspirin resistance in studied Iraqi patients was relatively high and risky so the specialist should be aware about such issue.
The presence of (CT) genotype of rs1035798 alone or with (CT) of rs184003 are associated with increase the likelihood for patient to be resistant to aspirin.