(Current Challenges and Updates in Pharmacy Education and Research)

Isolation, structural characterization and identification of quercitrin and essential oil in Cupressus sempervirens L. leaves growing in Iraq

Not scheduled

20m

كلية الصيدلة

Recorded poster Conference Track Three

Speaker

Amani A. Tawfeeq (College of Pharmacy, University of Baghdad, )

Description

Isolation, structural characterization and identification of quercitrin and essential oil in Cupressus sempervirens L. leaves growing in Iraq

Abstract
Cupressus sempervirens L., Cupressaceae, commonly known as evergreen cypress, Mediterranean cypress and in Arabic called “al -Sarw. it is an evergreen, medium sized, longevity, and wide distributed over all the world. C. sempervirens represents an important member of conifer plants which characterized with aromatic leaves and cones. Cupressus sempervirens have been ethnobotanical uses as an antiseptic, relief of cough, astringent, antispasmodic, wound healing and anti-inflammatory. The aim of this work is phytochemical analysis, isolation and structural identification of Quercitroside (quercitrin) and essential oil in Iraqi C. sempervirens. Isolation of quercitrin was performed by preparative HPLC from n- butanol fraction that prepared and extracted from Cupressus sempervirens leaves using ultrasound probe extraction, the structural identification of isolated quercitrin done by FT-IR characterized bands at frequency range 3160 cm -1, 3414 -3340 cm -1 ,1651, 1056 cm -1 and 1288 cm -1 .Moreover, LC /MS /MS; molecular ion at 448 m/z and base peak m/z 301. Furthermore, isolation of essential oil using hydro-distillation and estimated by GC-MS, the results show a good essential oil yield 0.9% with interesting concentration of alpha-pinene 44%, carene10%, cedrol4.86%, and β- myrcene3.67%. Quercetin glycoside and essential oil considered an important phytochemical with good concentration in Iraqi cypress plant.