(Current Challenges and Updates in Pharmacy Education and Research)

Nigella Sativa Seed Oil Effectively Attenuates Cyclophosphamide-Induced Myelosuppression in Mice

Not scheduled

20m

كلية الصيدلة

Poster exhibition (in-person) Conference Trak Two

Speaker

Ms shahad saad (ministry of health /medical city)

Description

Abstract
Myelosuppression is one of the serious adverse effects of cancer chemotherapy that lead to life threatening febrile neutropenia. Cyclophosphamide (Cyc), a widely used anticancer drug, induces severe myelosuppression. Nigella sativa seed oil (NG oil), a well-known plant extract that widely used for various health conditions. This study aims to evaluate the myeloprotective activity of NG oil in cyclophosphamide-induced myelosuppression mice model. Myelosuppression induced by single IP injection of Cyc (200 mg/kg). Animals were divided into 4 groups each with 6 mice. First group served as negative control group received only normal saline. A second group served as experimental myelosuppression model group achieved by Cyc. Additional 2 groups were mice received NG oil (1ml/Kg/day) or (2ml/Kg/day) orally for 6 consecutive days starting day 1 with Cyc on day 3. On day 7, blood were collected from retro–orbital area for total and differential leukocytes counts. Compared to model group, we reported that NG oil (1ml/Kg) significantly increases total leukocytes count (1000±73 vs. 700±36 cell/μL), lymphocyte count (868±63 vs. 580±40 cell/μL), spleen index (1.60±0.11 vs. 1.25±0.025 mg/gm), bone marrow cells viability (50.17±5.36 vs. 9.5±0.76%) and bone marrow GM-CSF (14.50±1.42 vs. 9.18±1.84 ng/l), while non significant change observed in neutrophil count and thymus index. Furthermore, increasing NG oil dose to (2ml/Kg) resulted in further improvement, were we reported a significant increase in total leukocytes count (1267±352 vs. 700±36 cell/μL), neutrophil count (279.67±90 vs. 95.5±8.4 cell/μL), lymphocyte count (1047±298 vs. 580±40 cell/μL), spleen index (2.35±0.49 vs. 1.25±0.025 mg/gm), thymus index (1.62±0.31 vs. 0.79±0.13 mg/gm), bone marrow cells viability (67.67±5.49 vs. 9.5±0.76%), and bone marrow GM-CSF (16.18±1.50 vs. 9.18±1.84 ng/l). In conclusion, we showed that NG oil has a promising strong myeoprotective and immunomodulatory effects against cyclophosphamide-induced myelosuppression.