Speaker
Description
Background:cell lines derived from cancer cells are frequently used in research, including use as a
model to understand cancer and to identify potential new treatments. The aim of this study has been
prepared to evaluate the anticancer effect of Rhus coriaria that has been studied for their
anticancer activity on esophagus and breast cancer cell line. Method: AMJ13(new breast cancer cell
line (AMJ13) has been established from an Iraqi breast cancer patient and SK-GT-4 cells (Human
esophageal adenocarcinoma cell line) were treated withethyl acetate fraction ofleavesand fruit
parts of plant extractsofRhus coriaria. the MTT assay to determine the anticancer activity was
done using 96-well plates where cell lines were seeded at 1 × 104cells/well after 24 hrs. the cells were
treated with tested compounds at different concentrations. Cell viability was measured after 72 hrs of
treatment by removing the medium, adding 28 µL of 2 mg/mL solution of MTT, and incubating the
cells for 2.5 h at 37 °C. After removing the MTT solution, the crystals remaining in the wells were
solubilized by the addition of 130 µL of DMSO (Dimethyl Sulphoxide) followed by 37 °C
incubation for 15 min with shaking. The absorbency was determined on a microplate reader at 492
nm. Conclusion: ethyl acetate fraction from Rhus coriaria showed particularly
strong anticancer capabilities since it inhibited actual tumor progression in a breast adenocarcinoma
mouse model. Our results suggest that ethyl acetate fraction of plant extracts are promising
anticancer reagents that has been studied for their anticancer activity on esophagus and breast cancer
cell line.
| Intend to be published in the conference journal (IJPS)? | No |
|---|---|
| Has the manuscript been published? | Published |
| Field/discipline | pharmacognosy |
