Speaker
Description
ABSTRACT
This work, for the first time, lupeol proof of existence as a novel metabolite in the Iraqi Leucaena leucocephala (Fabaceae) was the mainstay issue by identification of different phytochemicals compounds in the aerial Iraqi Leucaena leucocephala tree parts (leaves and stems) by using Gas Chromatography/Mass Spectrometry (GC/MS) technique and according to the part of the plant used and the method of extraction (hot and cold). This study made a comparison in lupeol concentration identified and isolated from petroleum ether extracted fractions of Leucaena leucocephala by using Gas Chromatography/Mass Spectrometry (GC/MS), High-performance thin-layer chromatography (HPTLC) and Preparative High-Performance Liquid Chromatography (P-HPLC). The plant leaves and stems were collected in September, dried under shade, and powdered (separately), then extracted by two extraction methods: hot Soxhlet and cold maceration method using 85% ethanol, then the result crude extract was fractionation with petroleum ether by using a separator funnel. The results of GC/MS, HPTLC and PHPLC indicated that the leaves contain a higher concentration of lupeol than the stems and the cold maceration method is more efficient than the hot Soxhlet extraction method. Lupeol has many pharmacological activities applied in alternative medicine such as anti-inflammatory, antimicrobial, anti-arthritic, anticancer, antidiabetic and antioxidant activities with wide future applications.
| Field/discipline | pharmacognosy |
|---|---|
| Intend to be published in the conference journal (IJPS)? | Yes |
| Has the manuscript been published? | Not published |
