Abstract
Abstract
Background: Daunorubicin (DNR) clinical utility is severely restricted by dose-dependent acute kidney injury.
Aim: This study evaluated and compared the protective effects of TND1128, a novel 5-deazaflavin derivative, against DNR-induced nephrotoxicity in male rats using lutein as a reference standard.
Methods: Male Sprague-Dawley rats were divided into six cohorts (n=6per group): control, DNR alone, TND1128 alone (10mg/kg/day) oral, 14 days), lutein alone (100 mg/kg/day oral, 14 days), TND1128 + DNR, and lutein + DNR. Intraperitoneal DNR (12 mg/kg/day) was given during the final three days to induce kidney injury. Serum markers (urea, creatinine), tissue endpoints (KIM-1, GSH, cleaved caspase-3, Nrf2), inflammatory gene expression (NF-κB IL-6), and renal histology were analyzed.
Results: DNR caused significant (P < 0.05) increases in serum urea, creatinine, KIM-1, cleaved caspase-3, and inflammatory genes (NF-κB, IL-6), alongside depleted antioxidants (GSH, Nrf2) and marked histological damage (tubular swelling, necrosis, vascular congestion). Prophylactic administration of TND1128 or lutein significantly attenuated all biochemical, molecular, and structural abnormalities.
Conclusion: TND1128 and lutein exert robust nephroprotective effects against DNR-induced acute kidney injury.
| Has the manuscript been published | not published |
|---|---|
| Intend to be published in the IJPS ? | Yes |
