Abstract
Natural remedies are increasingly being used to cure and prevent infectious diseases,
particularly those caused by multidrug-resistant (MDR) pathogens. These days,
medication resistance is a worldwide issue. As a result, having medications on hand that
can combat MDR infections is crucial. The spice clove (Syzygium aromaticum) is wellknown
for its many biological qualities. Eugenol is the main active ingredient in its
essential oil (EO), which also contains other active compounds. However, eugenol's
combined action with other components is responsible for the oil's biological effects.
The purpose of this study is to examine how eugenol-glycyrrhizic acid extract nanolipid
carriers (EGAE-NCL) can combat MDR bacteria that cause infections, illnesses, or
problems in humans, including Candida albicans, Staphylococcus aureus, and
Pseudomonas aeruginosa. High shear force homogenization was used to create EGAENLC
nanoparticles, which contained clove oil, licorice extract (glycyrrhizic acid), and
clove extract (eugenol). SEM and HPLC were used to verify that EGAE-NLC
nanoparticles were crystalline and pure. We concluded that the produced EGAE-NLC
nanoparticles were crystalline in nature based on the SEM data. The average size of the
EGAE-NLC nanoparticles was between 29 and 71 nm, and they demonstrated strong
antibacterial activity. The research results demonstrated EGAE-NLC nanoparticles can
inhibit the growth of MDR isolate S. aureus, P. aeruginosa and C. albicans.
